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	Provedor de dados BJM (8) Scientia Agricola (4) PAB (4) BABT (3) Rev. Microbiol. (2) Anais da ESALQ (1) Ciência e Agrotecnologia (1) Ciência Rural (1) Mais... Autor Melo,Itamar Soares de (24) Andreote,Fernando Dini (2) Dias,Armando Cavalcante Franco (2) Faull,Jane L. (2) Quecine,Maria Carolina (2) Silva,Célia Maria Maganhotto de Souza (2) Vieira,Rosana Faria (2) Zucchi,Tiago Domingues (2) Abakerli,Rosangela Blotta (1) Almeida,Jaqueline Raquel de (1) Azevedo,João Lucio de (1) Azevedo,Ricardo Antunes (1) Batista,Luis Roberto (1) Bettiol,Wagner (1) Carneiro,Paulo Irajara Borba (1) Carvalho,Giselle de (1) Cassiolato,Ana Maria R. (1) Castro,Renata Assis (1) Costa,Cyro Paulino da (1) Costa,Francisco Eduardo Carvalho (1) Mais... Palavra-chave Bacillus (3) Biological control (3) Biocontrol (2) Controle biológico (2) Endophytic bacteria (2) Trichoderma (2) 16S rRNA (1) Acinetobacter baumannii (1) Actinobacteria (1) Actinomycete (1) Alface (1) Alginato (1) Alternaria alternata (1) Alternative control of fungi (1) Antifungal activity (1) Auxotrophic mutants (1) Bacteria (1) Bacterial diversity (1) Benomil (1) Benomyl (1) Mais... Tipo do documento Info:eu-repo/semantics/article (22) Info:eu-repo/semantics/other (2) Ano 2018 (1) 2016 (1) 2014 (2) 2013 (1) 2012 (3) 2009 (1) 2007 (3) 2006 (2) 2004 (1) 2003 (1) 2000 (2) 1999 (5) 1987 (1) Mais... País Brazil (24) Idioma Inglês (17) Português (7)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 24 Primeira ... 1 2 ... Última Antagonism of Serratia marcescens towards Phytophthora parasitica and its effects in promoting the growth of citrus BJM Queiroz,Brigida Pimentel Villar de; Melo,Itamar Soares de. Phytophthora parasitica causes serious widespread, and difficult-to-control root rots in warmer regions. This oomycete is one of the most important pathogen of citrus. This paper reports the biological control of the pathogen by a strain of Serratia marcescens R-35, isolated from citrus rhizosphere. In greenhouse trials, the bacterium suppressed more than 50% of the disease and promoted the plant growth. Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Rhizobacteria; Plant growth promotion. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400008 Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a Scientia Agricola Melo,Flávia Mandolesi Pereira de; Fiore,Marli Fátima; Moraes,Luiz Alberto Beraldo de; Silva-Stenico,Maria Estela; Scramin,Shirlei; Teixeira,Manoel de Araújo; Melo,Itamar Soares de. In the search for new organisms and new secondary metabolites, a study was conducted to evaluate the diversity of endophytic bacteria from ethnovarieties of cassava cultivated by Brazilian Amazon Indian tribes and also to study the secondary metabolites produced by a Bacillus pumilus strain. Sixty seven cassava endophytic bacteria were subjected to 16S rRNA sequencing and FAME analysis. The bacterial profile revealed that 25% of all endophytic isolates belonged to the genus Bacillus. The isolate B. pumilus MAIIIM4a showed a strong inhibitory activity against the fungi Rhizoctonia solani, Pythium aphanidermatum and Sclerotium rolfsii. Secondary metabolites of this strain were extracted using hexane, dichloromethane and ethyl acetate. Extracts were subjected... Tipo: Info:eu-repo/semantics/article Palavras-chave: ESI-MS/MS; Pumilacidin; Endophytic bacteria; Bioautography. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000500002 Biological control of mycotoxin-producing molds Ciência e Agrotecnologia Medeiros,Flávio Henrique Vasconcelos de; Martins,Samuel Julio; Zucchi,Tiago Domingues; Melo,Itamar Soares de; Batista,Luis Roberto; Machado,José da Cruz. Mycotoxins are produced by the secondary metabolism of many fungi and can be found in almost 25% of the world's agricultural commodities. These compounds are toxic to humans, animals, and plants and therefore, efforts should be made to avoid mycotoxin contamination in food and feed. Besides, up to 25% of all harvested fruits and vegetables are lost due to storage molds and/or mycotoxin contamination and many methods have been applied to mitigate these issues, but most of them rely on the use of fungicides. Although chemicals are often the first defensive line against mycotoxigenic fungi, the indiscriminate use of fungicides are awakening the public perception due to their noxious effects on the environment and human/animal health. Thus, there is an... Tipo: Info:eu-repo/semantics/article Palavras-chave: Biocontrol; Microbial antagonists; Postharvest decay; Food safety. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000500001 Biological control of phytopathogenic fungi by endophytic actinomycetes isolated from maize (Zea mays L.) BABT Costa,Francisco Gheler; Zucchi,Tiago Domingues; Melo,Itamar Soares de. This work aimed a survey on the biodiversity of maize endophytic actinomycete, and an evaluation of their potential to control the phytopathogenic fungi. From several regions of São Paulo state, 40 strains were isolated from the healthy maize plants. The identification of these strains, based on morphological properties and fatty acid methyl ester (FAME) profile showed that most of them belonged to the Streptomyces genus. These isolates were first screened for the growth inhibition of phytopathogenic fungi and results showed that all the isolate were able to inhibit the development of at least one tested pathogen. Two selected isolates were then evaluated for the control of P. aphanidermatum in cucumber (Cucumis sativa L.) under greenhouse conditions.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytes; Actinomycete; Streptomyces; Pythium aphanidermatum; Dumping-off. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600009 Bioprospecting endophytic bacteria for biological control of coffee leaf rust Scientia Agricola Shiomi,Humberto Franco; Silva,Harllen Sandro Alves; Melo,Itamar Soares de; Nunes,Flávia Vieira; Bettiol,Wagner. Suppression of plant diseases due to the action of endophytic microorganisms has been demonstrated in several pathosystems. Experiments under controlled conditions involving endophytic bacteria isolated from leaves and branches of Coffea arabica L and Coffea robusta L were conducted with the objective of evaluating the inhibition of germination of Hemileia vastatrix Berk. & Br., race II, urediniospores and the control of coffee leaf rust development in tests with leaf discs, detached leaves, and on potted seedling of cv. Mundo Novo. The endophytic bacterial isolates tested proved to be effective in inhibiting urediniospore germination and/or rust development, with values above 50%, although the results obtained in urediniospore germination tests... Tipo: Info:eu-repo/semantics/article Palavras-chave: Hemileia vastatrix; Coffea spp.; Bacillus; Biocontrol. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162006000100006 Changes in hyphal morphology due to chitosan treatment in some fungal species BABT Oliveira Junior,Enio Nazaré de; Melo,Itamar Soares de; Franco,Telma Teixeira. In this work, changes in the hyphal morphology due to chitosan treatment in some fungal species were studied. Scanning electron microscope (SEM) observations revealed that chitosans with molar fraction of acetyl groups (F A 0.16 and 0.18) and degree of polymerization (DP 1,089 and 1,242) had a direct effect on the morphology of the chitosan-treated fungi, reflecting its potential for causing a delay in the growth of Alternaria alternata (500 µg × mL-1), Botrytis cinerea (1,000 µg × mL-1), Penicillium expansum (1,000 µg × mL-1) and Rhizopus stolonifer (500 µg × mL-1). Mycelial aggregation and structural changes such as excessive branching, swelling of the cell wall and hyphae size reduction were observed in the micrographs. Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitosan; Alternative control of fungi; Antifungal activity; Plant pathogenic fungi; Fungal morphology. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500001 Controle biológico da murcha de berinjela causada por Verticillium albo-atrum Anais da ESALQ Melo,Itamar Soares de; Costa,Cyro Paulino da. O controle biológico de Verticillium albo-atrum Reinke & Berth., agente causai da murcha de berinjela (Solanum melongena L.), foi feito em condições de laboratório e em condições de campo. Os melhores isolados de Trichoderma foram aqueles que apresentaram, em placas de Petri, uma zona de inibicão bem evidente. Trichoderma hamatum e o isolado T3 de Trichoderma reduziram sensivelmente a incidência da doença em condições de casa-de-vegetação. No campo, T. lignorum e o isolado T3 propiciaram os melhores resultados. Os mutantes de Trichoderma, obtidos por irradiação ultravioleta, não foram eficientes no biocontrole de Verticillium. Tipo: Info:eu-repo/semantics/article Palavras-chave: Verticillium albo-atrum; Trichoderma; Solanum melongena; Isolados. Ano: 1987 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0071-12761987000200022 Effects of different osmolarities on bacterial biofilm formation BJM Kavamura,Vanessa Nessner; Melo,Itamar Soares de. Biofilm formation depends on several factors. The influence of different osmolarities on bacterial biofilm formation was studied. Two strains (Enterobacter sp. and Stenotrophomonas sp.) exhibited the most remarkable alterations. Biofilm formation is an important trait and its use has been associated to the protection of organisms against environmental stresses. Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacteria; Biofilm formation; NaCl; D-sorbitol. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200034 Evaluation of bacterial diversity recovered from petroleum samples using different physical matrices BJM Dellagnezze,Bruna Martins; Vasconcellos,Suzan Pantaroto de; Melo,Itamar Soares de; Santos Neto,Eugênio Vaz dos; Oliveira,Valéria Maia de. ABSTRACT Unraveling the microbial diversity and its complexity in petroleum reservoir environments has been a challenge throughout the years. Despite the techniques developed in order to improve methodologies involving DNA extraction from crude oil, microbial enrichments using different culture conditions can be applied as a way to increase the recovery of DNA from environments with low cellular density for further microbiological analyses. This work aimed at the evaluation of different matrices (arenite, shale and polyurethane foam) as support materials for microbial growth and biofilm formation in enrichments using a biodegraded petroleum sample as inoculum in sulfate reducing condition. Subsequent microbial diversity characterization was carried out... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial diversity; Microbial enrichment; Petroleum reservoir; Physical support; 16S rRNA. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300712 Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum rifai Rev. Microbiol. Cassiolato,Ana Maria R.; Melo,Itamar Soares de. The isolation of genetic markers, like drug resistance and auxotrophy, is a laborious but important step in genetic research. The isolation of auxotrophic mutants of Trichoderma harzianum using the filtration enrichment technique was more effective than using the total isolation technique. Most of 12 auxotrophic mutants exhibited similar growth rate and higher sporulation when compared with the wild type, but only two mutants (TWS-410 and TW5-523) could grow in 500µg/L of benomyl. Tipo: Info:eu-repo/semantics/article Palavras-chave: Filtration enrichment technique; Auxotrophic mutants; Trichoderma harzianum; Benomyl. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000100008 Formulação do fungo Coniothyrium minitans para controle do mofo-branco causado por Sclerotinia sclerotiorum PAB Moretini,Alex; Melo,Itamar Soares de. O fungo Sclerotinia sclerotiorum, agente causal do mofo-branco, tem controle dificultado pela longevidade de seus escleródios no solo. Uma estratégia alternativa de controle é o uso do fungo antagonista Coniothyrium minitans, que parasita os escleródios de S. sclerotiorum e reduz a incidência da doença. O objetivo deste trabalho foi desenvolver uma formulação com C. minitans capaz de controlar o mofo-branco. Para tanto, picnídios deste fungo foram encapsulados com diferentes polímeros (alginato de sódio e pectina cítrica), caulim e substratos naturais (farinha de trigo e celulose). Das combinações obtidas, a formulação que continha 0,5% de alginato, 1,5% de celulose e 5% de caulim apresentou os melhores resultados quanto à viabilidade do fungo e controle... Tipo: Info:eu-repo/semantics/article Palavras-chave: Controle biológico; Fitopatógenos; Alface; Alginato. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000200003 Interspecific variation of the bacterial community structure in the phyllosphere of the three major plant components of mangrove forests BJM Dias,Armando Cavalcante Franco; Taketani,Rodrigo Gouveia; Andreote,Fernando Dini; Luvizotto,Danice Mazzer; Silva,João Luis da; Nascimento,Rosely dos Santos; Melo,Itamar Soares de. Mangrove forests encompass a group of trees species that inhabit the intertidal zones, where soil is characterized by the high salinity and low availability of oxygen. The phyllosphere of these trees represent the habitat provided on the aboveground parts of plants, supporting in a global scale, a large and complex microbial community. The structure of phyllosphere communities reflects immigration, survival and growth of microbial colonizers, which is influenced by numerous environmental factors in addition to leaf physical and chemical properties. Here, a combination of culture-base methods with PCR-DGGE was applied to test whether local or plant specific factors shape the bacterial community of the phyllosphere from three plant species (Avicenia... Tipo: Info:eu-repo/semantics/article Palavras-chave: Culture-independent profiling; Plant genotype; Surface leaves. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200030 Isolamento de fungos degradadores de carbendazim PAB Silva,Célia Maria Maganhotto de Souza; Melo,Itamar Soares de; Maia,Aline de Holanda Nunes; Abakerli,Rosangela Blotta. Os fungicidas sistêmicos do grupo dos benzimidazóis são amplamente utilizados no controle de muitas doenças de plantas, tanto no solo como na parte aérea, e isso tem sido causa de contaminação ambiental. A ação de alguns microorganismos contribui para degradação e perda da atividade biológica desses fungicidas, podendo reduzir, dessa forma, o risco de impactos negativos. O objetivo deste trabalho foi selecionar fungos com potencial para degradar benomil e seu produto de hidrólise, carbendazim, e quantificar o potencial de degradação. Fungos foram isolados de três diferentes solos da região de agricultura irrigada do município de Guaíra, SP, com histórico de aplicação intensiva de fungicidas benzimidazóis. Dentre os fungos isolados, Alternaria alternata foi... Tipo: Info:eu-repo/semantics/article Palavras-chave: Degradação; Alternaria alternata; Benzimidazóis; Benomil. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X1999000700019 Isolamento e caracterização de bactérias degradadoras do herbicida diuron Scientia Agricola Roque,Milton Ricardo de Abreu; Melo,Itamar Soares de. Solos provenientes de áreas cultivadas com cana-de-açúcar, e histórico de utilização do herbicida diuron, foram utilizados para isolamento e caracterização de linhagens bacterianas com potencial de degradar este herbicida. As linhagens foram isoladas de solos da rizosfera de cana-de-açúcar e de solos das entre linhas. Noventa e oito linhagens foram isoladas, das quais 27 foram selecionadas para avaliação do crescimento em meio mineral sólido suplementado com diuron nas concentrações de 5, 10 e 20 mig mL-1. Neste experimento, 5 linhagens apresentaram um crescimento exponencial entre 0-48 horas (EMBRAPA-CNPMA D12-12, D12-14 e D16-12) e entre 0-110 horas (EMBRAPA-CNPMA D11-9 e D12-18), similar ao controle. A linhagem EMBRAPA-CNPMA D12-12 identificada como... Tipo: Info:eu-repo/semantics/article Palavras-chave: Acinetobacter baumannii; Biodegradação; Herbicidas; Curva de crescimento. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000400020 Mangrove endophyte promotes reforestation tree (Acacia polyphylla) growth BJM Castro,Renata Assis; Dourado,Manuella Nóbrega; Almeida,Jaqueline Raquel de; Lacava,Paulo Teixeira; Nave,André; Melo,Itamar Soares de; Azevedo,João Lucio de; Quecine,Maria Carolina. ABSTRACT Mangroves are ecosystems located in the transition zone between land and sea that serve as a potential source of biotechnological resources. Brazil's extensive coast contains one of the largest mangrove forests in the world (encompassing an area of 25,000 km2 along all the coast). Endophytic bacteria were isolated from the following three plant species: Rhizophora mangle, Laguncularia racemosa and Avicennia nitida. A large number of these isolates, 115 in total, were evaluated for their ability to fix nitrogen and solubilize phosphorous. Bacteria that tested positive for both of these tests were examined further to determine their level of indole acetic acid production. Two strains with high indole acetic acid production were selected for use as... Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic bacteria; IAA; Phosphorus and plant growth promoting bacteria (PGPB). Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100059 Microrganismos endofíticos de mandioca de áreas comerciais e etnovariedades em três estados brasileiros PAB Teixeira,Manoel Araújo; Melo,Itamar Soares de; Vieira,Rosana Faria; Costa,Francisco Eduardo Carvalho; Harakava,Ricardo. O objetivo deste trabalho foi realizar um levantamento da diversidade de microrganismos endofíticos, em plantas de mandioca (Manihot esculenta Crantz) coletadas de áreas comerciais, no Estado de São Paulo, e de etnovariedades dos estados do Amazonas e Bahia e, também, avaliar seu potencial para fixar N atmosférico e para produzir ácido indolacético. Nos três estados, foram identificadas 47 espécies de microrganismos pertencentes a 27 gêneros. Bacillus spp. foi o mais freqüente em todas as regiões. O maior número de gêneros foi encontrado em plantas provenientes do Estado do Amazonas, que apresenta a maior diversidade de microrganismos endofíticos. Amplificações por PCR do gene nifH foram avaliadas em espécies bacterianas pertencentes às... Tipo: Info:eu-repo/semantics/article Palavras-chave: Manihot esculenta; Bacillus; Actinobacteria; Fixação de nitrogênio; Ácido indolacético. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000100006 Parasitism of Rhizoctonia solani by strains of Trichoderma spp. Scientia Agricola Melo,Itamar Soares de; Faull,Jane L.. Rhizoctonia solani causes serious diseases in a wide range of plant species. The fungus Trichoderma has been shown to be particularly effective in the control of the pathogen. Thus, this research was carried out to screen fourteen Trichoderma strains against R. solani in vitro. All strains tested inhibited the growth of R. solani. Three T. koningii strains produced toxic metabolites with strong activity against R. solani, inhibiting the mycelial growth by 79%. T. harzianum, Th-9 reduced the viability of sclerotia of R. solani by 81.8% and T. koningii, TK-5 reduced by 53%. Electron microscopic observations revealed that all T. harzianum strains interacted with R. solani. Th-9 grew toward and coiled around the host cells, penetrating and destroying the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhizoctonia; Trichoderma; Biological control. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000100010 Production of phenol-oxidases and peroxidases by fungi isolated from irrigated rice BJM Silva,Célia Maria Maganhotto de Souza; Melo,Itamar Soares de; Vieira,Rosana Faria. The aim of this work was to study the potential of fungus strains considered as prospective degraders for the herbicides quinclorac and propanil, for ligninolytic enzyme production. Eight fungal strains were grown in King's B liquid culture medium supplemented with 0.05% Remazol Brilliant Blue R (RBBR) and in liquid culture medium containing wheat bran as substrate. The enzymatic system assessment were: lignin peroxidase, manganese peroxidase and laccases. Results indicated differential patterns of ligninolytic enzyme production; the highest enzymatic activities were related to the production of lignin peroxidase. Among the strains, two (P3SA1F and P11SA2F) showed RBBR discoloration, suggesting the possibility of their application in bioremediation studies. Tipo: Info:eu-repo/semantics/article Palavras-chave: Ligninolytic enzymes; Laccases; Remazol Brilliant Blue R. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500018 Requisitos nutricionais para o fungo Alternaria alternata PAB Silva,Celia Maria Maganhotto de S.; Melo,Itamar Soares de. Uma linhagem de Alternaria alternata (ALT A) foi cultivada em meio sintético com diferentes fontes de C, N e vitaminas. O crescimento micelial foi avaliado durante sete dias após a semeadura e a esporulação no décimo dia de incubação. O meio sintético básico suplementado com biotina, NH4Cl e os carboidratos maltose e glicose suportaram um bom crescimento micelial (em média 0,75 cm/dia). Galactose e arabinose induziram significativamente a produção de esporos (mL-1), da ordem de 5,0 x10³ e 4,1 x 10³, respectivamente. Nas diferentes fontes de vitamina e N não houve diferenças significativas quanto ao crescimento micelial e à esporulação. Tipo: Info:eu-repo/semantics/article Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X1999000300024 Scanning electron microscopy of conidia of Trichoderma stromaticum, a biocontrol agent of witches' broom disease of cocoa BJM Melo,Itamar Soares de; Faull,Jane L.. A field emission electron microscope (SEM) was used to study the conidia surface of Trichoderma stromaticum, a biocontrol of witches broom disease of cocoa. Surface features of conidia were difficult distinguish by light microscope. Conidia appeared to be verrugose and minutely roughened, but the nature of the roughening was not easy to discern. It was common to observe sheath-like structures that completely covered groups of conidia, and also details of wide cells that form the pustules. Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Cocoa; Crinipellis pernisiosa; Morphology of conidia. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300011 Registros recuperados: 24 Primeira ... 1 2 ... Última

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